Device

Part:BBa_K2874001

Designed by: Rishabh Jain   Group: iGEM18_IIT_Kanpur   (2018-10-09)


JA105->PelB-sfGFP-His tag

This part was designed to test the efficiency of extracellular expression of proteins using pelB tag under JA105 promoter(Bba_J23105). We have incorporated super folded Green fluorescent protein(sfGFP) along with his tag to detect extracellular expression of the protein.

We have also added a Nru1 site before sfGFP sequence. This can be used for cloning of the protein of interest that an iGEM team wants to express extracellularly.

Thus, this plasmid provides a system for cloning of any protein of interest into the plasmid and using it for extracellular expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 113
    Illegal NgoMIV site found at 837
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None